![]() The Queen’s Gambit (2020)Ĭast: Anya Taylor-Joy, Chloe Pirrie, Bill Camp, Marcin Dorocinski A Netflix original reality TV show that keeps it real and interesting for you. The style experts forge relationships with men and women who frequently have different opinions from their results in social criticism interwoven with style guidance. Food and wine expert Antoni Porowski, interior designer Bobby Berk, grooming advisor Jonathan Van Ness, fashion designer Tan France, and cultural specialist Karamo Brown all offer advice to those in need of lifestyle makeovers. The "Fab Five" devote a week of each episode to using their knowledge to assist someone to improve their lifestyle and find greater happiness in life. The award-winning program is renowned for having a significant presence in both the LGBT community and communities with a population of colour. Queer eye (2018-present)Ĭast: Antoni Porowski, Karamo Brown, Bobby Berk, Jonathan Van NessĪn entertaining, well-liked Netflix reality series featuring the "Fab Five" men, switches out the concrete jungle of New York City for other areas of the United States. But with so many references to sex, drugs, and alcohol, this one of the highest-rated Netflix series is not appropriate for children. Only once you view it will you truly understand what a masterpiece this is. The fact that a horse is one of the most human characters on television is a wonderful irony, and the show's unwavering focus on the character of the horse makes it the greatest. This is a program that faces some extremely harsh facts of life directly in the eye and goes beyond visual gags, crude dialogue, or delightfully cheap laughs. The title sequence shows BoJack's sad slide from being the comedy star of the popular television show "Horsin' Around" in the 1980s and 1990s to being drunk in Hollywood, whining about everything, and wearing bright sweaters. This is one of the most intelligent comedies ever produced. TompkinsīoJack Horseman is one of the most high-rated animated comedies ever made on Netflix. BoJack Horseman (2014-2020)Ĭast: Will Arnett, Aaron Paul, Amy Sedaris, Paul F. The ultimate list of highest-rated Netflix series of all time 1. Well, look no further, we have put together a list of the best highest-rated Netflix original series of all time for you. ![]() But with so many choices, it might be difficult to choose which series to watch. ![]() Synopsis- A link in their pasts leads an honest cop to a fugitive gang boss, whose cryptic warning spurs the officer in a quest to save Mumbai from the cataclysm.Nevertheless, Netflix offers a tonne of top-notch TV series, with a new season premiering every week, so you can binge-watch them anytime you choose.Starring- Saif Ali Khan, Nawazuddin Siddiqui & others.Here is the list of the Top 50 Most Popular Indian Web Series of All Time 1. Meanwhile, this list can prove to be a checklist or a binge-watch list for those who love to consume content and are looking for something good to watch these days. It reinforces our faith that we are doing something right and further fuels our motivation to work hard and do our best and make good cinema.” Speaking about two of their web series (Farzi & The Family Man) being included in the list, Raj and DK shared, “We are flattered and humbled that both of our shows, ’The Family Man’ and ‘Farzi’ have made it into the list. The rankings of the Top 50 Most Popular Indian Web Series of all time are determined by the page views of IMDb customers in India between Januto May 10, 2023.” IMDb itself shared the video, with the caption- “The IMDb Top 50 Most Popular Indian Web Series of All Time are here! Save this post to complete your watchlist. The list includes names like Shahid Kapoor’s Farzi, Manoj Bajpyaee’s The Family Man, and Vikrant Massey’s Broken But Beautiful & Criminal Justice.īefore we share the list of these top 50 web series, let us have a look at how the list was created and how do those included in the list feel. The list includes shows streaming on Netflix, Disney+Hotstar, Amazon Prime, Zee5, SonyLIV, MX Player, Voot, and Jio Cinema (a total 12 OTT platforms). ![]() Now, IMDb has released a list of the 50 most popular Indian web series of all time. One of the most common parameters to measure the success of a web show is its IMDb Rating. However, no matter how much content is released, only some shows are able to get love from the audiences, while others fade away into oblivion. Whether they are in English or in Hindi, whether they belong to the genre of thriller or romance, there is no dearth of content to stream online. With OTT Streaming on an all-time rise, various leading platforms stream a multitude of web series every other day. ![]()
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![]() ![]() Note: This API call contains a body in JSON formatģ. Select the API call to execute, in our case is Tip: If you wish to explore other API calls, please go to vManage url 2.Proceed to execute another API to vManage. Warning: If you did not get a token as shown in the image please repeat the step. The image shown exemplifies the output In Postman. Once the API call is executed, you get a response string in the body. Note: Since vManage version 19.2.1, it has been made mandatory that a successfully logged in user needs to send X-XSRG-TOKEN or CSRF token for each POST/PUT/DELETE operation via API call. Note: As username and password values, we use admin.Ĭaution: The response of this API call must be empty Step 3. Note: In this example vManage ip address is 10.88.244.30 and the port is 5301 Click Body and add as KEY parameters j_username and j_password and their values respectively.Authenticate with your username and password credentials to vManage. You can create a new HTTP requests if you click in any of the highlighted options.Ĭreate a new HTTP request. Open Postman and create a new HTTP request. ![]() Configure Postman to Execute the APIs Step 1. Of those less-frequent methods, OPTIONS and HEAD are used more often than others. There are a number of other verbs, too, but are utilized less frequently. These correspond to create, read, update, and delete (or CRUD) operations, respectively. The primary or most-commonly-used HTTP verbs (or methods, as they are properly called) are POST, GET, PUT, PATCH, and DELETE. Note: If you do not have Postman, download it from Background Information Access to vManage and username and password credentials. ![]() This document describes how to execute Application Programming Interfaces (APIs) with Postman. ![]() ![]() ![]() So, what exactly does this recipe entail? TikTok’s Toscanas Table, a popular meals and recipe account, took a healthy dessert and elevated it to new heights. Will I continue savoring this dessert to help me drift off to sleep, or will I stick to my trusty melatonin gummies? Let’s discover if this cherry dessert helped with those restless nights! What Is The “Sleepy Girl Sorbet?” I decided to test this delectable dessert for three consecutive nights to determine if it truly lives up to its reputation.įrom past TikTok trends such as the sleepy girl mocktail and drinking tart cherry juice, the properties of cherries demonstrate that it can help us get a little sleepy when needed. However, I desired to combine my beloved late night snacks with something that might be able to sleep. When I struggle to sleep, I resort to taking melatonin. So, when I came across a novel sorbet recipe on TikTok, I felt compelled to give it a shot. Among my favorites are dark chocolate-coated strawberries and blueberries. The groups were mutual fans of each other, and he took on the early Basement Screams-era deep cut “Got Hurt” partially because it had only been released as a work-in-progress bonus track.If there is one thing you should know about me, it’s my love for enjoying a delightful treat before bedtime. Robbins, whose connection to Naked Raygun dates back to his mid-80s stint in the Washington, D.C. Your first taste of the compilation comes from Jawbox frontman and punk lifer J. “ Naked Raygun are one of the few bands that became a permanent landmark in how we, as a band, see and approach the music we play” Chris Wollard, Hot Water Music You can feel how much Kezdy meant to this scene. In one of the compilation’s many standout moments, Pegboy honors their “perfect bandmate and dear friend” with a powerful, soul-shaking cover of “Vanilla Blue” from 1988’s Jettison. Since Kezdy’s greatest impact was here in Chicago, it’s only fitting that some of the album’s highlights come from locals, like The Bollweevils, The Usuals, The Methadones and Smoking Popes frontman Josh Caterer. All of the profits go directly to Kezdy’s family to help them continue to honor his legacy and creativity. Robbins, Hot Water Music and Swingin’ Utters covering their favorite Naked Raygun classics. So what better way to celebrate Kezdy’s life than by remembering all of the incredible music he gave us over the years?Īvailable digitally and on vinyl July 28, Godspeed… A Tribute to Pierre Kezdy features artists like J. It’s no wonder why bands like Foo Fighters, Fall Out Boy, The Lawrence Arms and blink-182 cite Naked Raygun as an essential part of their punk rock education. He also took great pride in Naked Raygun’s influence on other artists, telling the Chicago Ambassador, “We were the guys out there with machetes, blazing a path through the jungle while other people were able to follow and bring their weapons through easily.”Ĭombining the intensity of hardcore with melodic sensibility, Naked Raygun helped punk grow into something bigger, broader and more creative. ![]() ![]() He passed away on Octoat the age of 58.įans will always remember Kezdy doing what he loved most, standing stage left in a power stance with that black, sticker-covered Gibson bass slung over his shoulder. Kezdy experienced a number of health issues, including a stroke in 2011 and a cancer diagnosis a few years later. While there was plenty to celebrate, these years weren’t without hardship. Among the band members was bassist Pierre Kezdy, whose playing was a driving force in the band’s sound. For thirty minutes on that rainy, muggy August night in 2015, Naked Raygun embraced stadium rock. More than three decades later, Grohl would invite his heroes to open for Foo Fighters at Wrigley Field. Little did I know that all you needed was four walls and a song.” Dave Grohl ![]() “I foolishly thought that bands performed only on giant stages with smoke machines and massive displays of lasers and pyrotechnics. In his memoir, The Storyteller, Grohl reflected on the stripped-down Naked Raygun show. Hell, he’d never been to a show before! That Naked Raygun set was his big bang moment, a day that would set him on a trajectory to book his own shows, conquer MTV, tour the world, win 18 GRAMMY Awards, get inducted into the Rock & Roll Hall of Fame twice and, in 2023, headline Riot Fest. Until that day, he didn’t know the first thing about punk rock. For almost everyone involved, this was just another gig.īut, for a scrawny 13-year-old boy from Virginia, this was everything. The Wrigleyville dive was known for its all-ages matinees featuring punk and hardcore bands like Hüsker Dü, T.S.O.L. It was the summer of 1982, and Naked Raygun was playing at the Cubby Bear. ![]() ![]() The Brutus V9 weighs 780 pounds (354 Kg). ![]() Still, its battery and fairing put this bike in the upper-heavyweight class of electric motorcycles. ![]() The V9 electric battery is no joke, offering 270 miles at an average speed between charges. While it’s got a governed top speed of 115 mph, the Brutus V9 boasts a Horsepower of 125 and a pavement-crushing 277 foot-pounds of torque. The big-bad bagger called Brutus is one of the only electric bikes with a classic, tough-bike character about it. The Lightning LS-218 weighs 495 pounds (225 Kg). Lightning LS-218Īs the name implies, this electric enigma right here can clear 218 mph.Ĭonsidered the fastest electric-bike to date, the LS-218 roasts it’s way from 0-60 in 2.2 seconds with a 12 kWh battery pack under the seat. The Victory Empulse TT weighs in at 470 pounds (213 Kg). The name’s been quiet, but the AC Magnet Motor in this little nugget of theirs makes it one of the best performers on the list, delivering heavy power for midweight. Polaris’ pre-curser to reviving Indian Motorcycles was to use their world-class engineering to launch their Victory Motorcycle brand back in 1998. You may also be interested in our article: where are Harley Davidson’s made? 2. The Harley Davidson Livewire weighs 549 pounds (249 Kg). Their all-electric LiveWire has an industry-leading electric motor that rips from 0-60 in 3 seconds and goes 146 miles per charge, putting the bike right in the middle of the list that ranges from light to mid to heavyweights. Harley Davidson has managed to stay in front of the ever-shifting bike trends for over a hundred years, and while the brand is known for consistency in style and performance, they’re no strangers to taking chances. Weight of Various Electric Motorcycles Electric Motorcycle ![]() Electric Motorcycles of their Own Class.Alta Motors Redshift MX Electric Dirt Bike ![]() ![]() ![]() SNAP's share price has fallen by -34% from $75.11 as of Octoto $49.26 as of Novemin the past one month. Given that it is difficult to determine when Snap Inc.'s revenue growth momentum can recover, it is tough to have a positive view of the company's shares at the moment, which makes a Neutral rating fair. Snap Inc.'s Enterprise Value-to-Revenue multiple has come down to close to its three-year historical average, but the stock's revenue growth expectations are also more modest now. With the market having less confidence in Snap Inc.'s ability to generate strong revenue growth in line with the goals it set at its February 2021 Investor Day, SNAP's valuations have de-rated significantly in line with a substantial share price correction in the last one month. In the current article, I review Snap Inc.'s third quarter earnings and assess its future financial outlook, and determine if the stock is attractive as a potential investment candidate now.Īpple's ( AAPL) iOS privacy changes have led to SNAP's Q3 2021 actual revenue and its Q4 2021 revenue guidance coming in below market expectations. My prior update for SNAP was published on Augafter the company reported its Q2 2021 results. ![]() ( NYSE: SNAP) following the company's Q3 earnings drop and subsequent stock price correction. I maintain my Neutral rating for Snap Inc. Justin Sullivan/Getty Images News Elevator Pitch Mark Hake writes about personal finance on and runs the Total Yield Value Guide which you can review here. The opinions expressed in this article are those of the writer, subject to the Publishing Guidelines. ![]() Hake did not hold any position (either directly or indirectly) in the securities mentioned in this article. Despite SNAP’s present troubles, expect SNAP stock to be 39% higher within two years from higher free cash flow. That is an excellent return on investment (ROI) for most investors. Even if it takes two years, the average compounded return will still be 17.9% for each of the next two years. Let’s assume that it takes over one year for this price target to hit. So this also implies that even with the lower Q4 estimates, the long-term target price for SNAP stock is $66.68 per share. This means that the $106.7 billion target market value is 39% higher than its $76.6 billion market cap today. But if we determine its future value by using the FCF yield metric - this is a rate that we can use to estimate how valuable its FCF is to the market.įor example, if we divide $1.6 billion in FCF by an FCF yield of 1.5%, the target market cap will be $106.7 billion (i.e, $1.5b/0.15=$106.7b). Right now, SNAP has a market capitalization of $76.64 billion. If revenue doubles to $8 billion by 2023, even with lower iOS ad sales, it is likely that FCF will be much higher as well.įor example, using a 20% FCF margin, we can estimate that FCF will hit $1.6 billion by 2023. These are the same analysts that were upset that that Q3 revenue came in below their forecasts.Īssuming that the company will produce free cash flow at a high margin in two years will allow us to to forecast the stock price. Analysts are still projecting revenue to hit $8 billion in 2023, up from $4.02 billion this year, according to Seeking Alpha. That does not seem too high for a company that will be growing revenue by over 100% by then. That puts SNAP stock on a forward price-to-earnings (P/E) valuation metric of just 35 times. As it stands now, SNAP stock’s valuation is starting to look interesting.įor example, these same analysts now project that earnings in two years will hit $1.35 per share at the end of 2023. Moreover, according to the Seeking Alpha, the Q4 guidance was well below (-13%) analysts’ expectations.īut this just explains why SNAP stock has fallen. For example, according to one analyst at Seeking Alpha, the company’s Q3 revenue was 3% below analyst expectations. Moreover, SNAP still projected that its Q4 revenue would be higher than Q3 revenue, albeit lower than analysts’ prior projections. More importantly, FCF became positive at $52 million in Q3 2021, compared to $70 million in the prior year. Analysts overlooked this in the Q3 earnings release.įor example, operating cash flow was $72 million in Q3 2021, compared to a loss of $55 million in Q3 2020. One reason is that SNAP is now making operating and free cash flow (FCF) profits. ![]() ![]() ![]() However, if you are looking to go for the record, it is currently at around 13 minutes and 30 seconds. Really skilled veterans of Moto X3M can speedrun the game in roughly 20 minutes if they know the maps well and have played the game for a while. What is considered a good Moto X3M speedrun? Each level includes several checkpoints, along with loops, turns, high-flying jumps, and opportunities to do plenty of flips and tricks. Moto X3M has 22 levels, each more action-packed than the last. For a more in-depth guide to Moto X3M strategies, feel free to check out our blog about it here. Sometimes it’s about being smooth, not speedy. While you’ll have to go super fast most of the time, there will be levels where slowing down is important to keep control of your bike. Try to flip as often as you can! Don’t be afraid to fail, and make sure to control your speed. There will be a different challenge with every level, so players should be ready to have anything and everything thrown at them. Some levels have giant loops, some have terrifying drops, some have moving walls that can crush you with ease. Besides that, there are tons of different traps and obstacles that you have to get through on each map. The only similarity throughout all of the levels is that they are fast-paced and will require a decent amount of skill to get 3 stars in. What’s different between Moto X3M levels? While at first, it may be easy to get 3 stars, the later levels will really push you to be perfect if you want to get 3 stars. It is purely based on the players’ time, and each level will have a certain time that players must beat if they hope to get 3 stars. Stars are given at the end of each level, and players are able to earn anywhere from 1 to 3 stars on every attempt. Just don’t get too crazy and land on your head, or else you’ll have to either restart the level or go back to the previous checkpoint. This means that front flips and backflips are heavily encouraged, and in some cases may even be necessary in order to get 3 stars. As previously mentioned, doing stunts in the air will shave off time. ![]() The more dangerous you play the game, the more you are rewarded. Moto X3M isn’t just made for anyone though, it’s made for the adrenaline junkies on our site who want to play something where they can test their skills and reactions. Kick up some dirt with this fast-paced racing game that is sure to get your heart pumping. Do stunts in the air to shave time off of your run! Make it through the course as quickly as possible. Use the arrow keys to accelerate, brake, or flip your bike. ![]() ![]() ![]() While the Mi Band "only" offers 30 different sports modes, the Huawei Band 6 will include sports such as skydiving, laser tag, or car racing. If the type of sporting activities that you pursue are more out of the ordinary, the Huawei Band 6 has the edge here. With these sensors, the Huawei Band 6 can take a peek under your skin! / © NextPit Oh yes, an ambient light sensor is also missing. This means that you should always have your smartphone nearby to keep track of your daily sports routine. While the Huawei Band 6 and the Mi Band 6 have sensors that detect your pulse, the oxygen content of your blood, acceleration sensors, and Bluetooth, both WLAN and GPS are missing. When you look at the specifications sheet, you will notice a lot of similarities in the sensors and features offered. Most certainly a perfect transition! Sensors and range of functions Despite the different manufacturers, you have a similar range of functions at your disposal. You navigate through their respective proprietary operating systems via swiping gestures. Once activated, operating both devices are rather similar. ![]() This does tip the Huawei Band 6 in favor of athletes* who often wear gloves or other sports gear that tend to get in the way of touchscreens. Xiaomi, on the other hand, dropped the capacitive control button below the display in the sixth generation. For activation, Huawei places a "real" button on the right side of the Huawei Band 6. True to the motto "form follows function," you operate both fitness trackers mainly via the touchscreen in front. Swimmers would definitely find it useful as both fitness trackers offer a swimming tracking mode.Īlthough Xiaomi has ditched the soft-touch button, the design remains round / © NextPit Both models are protected against water up to 5 ATM, making them suitable for use while swimming. Of course, the important thing when designing a fitness tracker is whether the case offers protection against water and dust penetration. ![]() ![]() The Huawei model tips the scales at 18 grams, making it even lighter than the 24 grams of the Mi Band 6. The Mi Band 6 measures 47.4 x 18.6 x 12.7 millimeters, while Huawei's tracker is significantly wider at 43 x 25.4 x 11.45 millimeters, albeit shorter and flatter. The Huawei Band 6 is a bit more angular in comparison / © NextPitĮven though the case sizes are by no means identical, both fitness trackers are very compact devices in their own right. Xiaomi's AMOLED display measures 1.56-inches, while the Huawei model packs a 1.47-inch AMOLED panel. However, the displays on both models are now larger compared to their respective predecessors. Design and operationįinally, two fitness trackers that are visually different from one another! Because while Xiaomi still relies on curves for the design of the Mi Band 6, the Huawei Band 6 sports corners and edges. Update: The Honor Band 6 received a firmware update on December 2021 that allows for automatic SpO2 monitoring. For this very reason, we picked the Huawei model for this comparison. But first, a little caveat: The Honor Band 6 might be a bit cheaper than the Huawei Band 6, but it doesn't offer SpO2 tracking 24 hours a day. In the following examination, will take a look at how the two fitness trackers compare. Thus, these models have been recommended as a favorable alternative if you are asthmatic or have to pay closer attention to your SpO2 value for other health reasons. Instead, models such as the Huawei Band 6 and the Xiaomi Mi Band 6 are also able to keep track of the oxygen content in your blood as well as your pulse rate. Cheap fitness trackers no longer only keep track of your 10,000 daily steps. ![]() ![]() Pairing him with Clady will afford the Broncos a bridge to a point in the future where Sambrailo can take over the starting left tackle position.Mitchell Schwartz is best known for playing Offensive tackle with the NFL team Kansas City Chiefs from 2016 to 2020. His teammates love him to the point where Joe Thomas thinks the Browns will be in dire straights if they let this guy go. Schwartz has proven durability and is still quite young. He definitely struggled as a rookie, though not nearly as much as those who filled in for him after Sambrailo was sent to injured reserve. It would also afford Ty Sambrailo some time as a backup to come in and learn the ropes. That's at least intriguing enough to me to want this contract restructure to work. If that happens, then the Broncos might have some wiggle room in the cap to target a guy like Schwartz who is projected to be one of the highest paid right tackles in the NFL.įor those of you who think Clady is done and should be discarded as soon as possible, let's compare his run blocking grades under the zone blocking scheme under Mike Shanahan to the power run scheme that John Fox implemented. Clady has seen his play degrade over the last three seasons due to injuries, but with a full year to heal heading into 2016 he could be an interesting option at left tackle if he is willing to restructure as reports have suggested. Why Schwartz is a good fit for the Broncos I don't know, the point is the Broncos can ill afford to enter the season with any of the three guys in that table that started down the stretch. Half of a Schwartz would have been enough to get the job done here. To demonstrate how bad things were and why it probably played a huge part in the Broncos offensive woes, let's look at the PFF grades for the tackles who played a majority of the season for the Broncos in 2015. That's right, every team in the NFL almost had two tackles that played better than the Broncos best tackle. He was also the best tackle on the Broncos roster, even though he graded out a little lower than Michael Schofield. In 2015, Ryan Harris was the 60th best tackle in the NFL as ranked by PFF. The situation is not as dire as it was a month ago, but it is clear that the team is still one injury away from disaster if they don't do something to improve the depth at the tackle position. At the right tackle position, the Broncos would be looking at a five year deal somewhere between $30-35 million according to Over the Cap. He should end up in the top five in the NFL in terms of pay at that position in a few weeks. All in all, Schwartz should command a large contract at the right tackle position. One underrated quality is his intelligence, having even taken a few agents to task over player contracts on Twitter. He is also remarkably durable having started all 64 games of his career. Schwartz is the fifth best tackle as rated by Pro Football Focus is also the best rated right tackle in this free agent class. Overall, he has only put up a single negative grade in any of his four seasons with an impressive cumulative grade in pass blocking (+30.9) and a respectable grade in run blocking (+4.9). Mitchell Schwartz was drafted in the second round by the Cleveland Browns in 2012 and has had himself a solid career so far, posting career high PFF grades in both pass blocking (+13.0) and run blocking (+3.7) in 2015. ![]() Let's take a look at one of the better options to bolster the offensive tackle position heading into the 2016 regular season. The tackle position is something John Elway is going to have to address in free agency and the 2016 NFL Draft. Though it was a disaster, the Denver Broncos somehow emerged with a Super Bowl 50 victory due almost entirely to the play of their otherworldly defense. From there, things only got worse as rookie Ty Sambrailo was also lost for the year leaving just Ryan Harris, Michael Schofield and Tyler Polumbus to hold the line. In 2015, the offensive tackle position was maligned from almost day one when Ryan Clady went down for the season. ![]() ![]() ![]() In a crucial step toward single-nucleotide-resolution nanopore sequencing, engineering of the wild-type α-hemolysin protein allowed the four DNA bases on oligonucleotide molecules to be distinguished, although complex sequences were not examined in these reports 13, 14, 15. 1, 9), was the first nanopore shown to detect recognizable ionic current blockades by both RNA and DNA homopolymers 10, 11, 12. α-Hemolysin, a membrane channel protein from Staphylococcus aureus with an internal diameter of ~1.4 nm to ~2.4 nm (refs. The concept of nanopore sequencing emerged in the 1980s and was realized through a series of technical advances in both the nanopore and the associated motor protein 1, 4, 5, 6, 7, 8. As nucleotides pass through the nanopore, a characteristic current change is measured and is used to determine the corresponding nucleotide type at ~450 bases per s (R9.4 nanopore). Under the control of a motor protein, a double-stranded DNA (dsDNA) molecule (or an RNA–DNA hybrid duplex) is first unwound, then single-stranded DNA or RNA with negative charge is ratcheted through the nanopore, driven by the voltage. Ionic current passes through the nanopore because a constant voltage is applied across the membrane, where the trans side is positively charged. Each channel associates with a separate electrode in the sensor chip and is controlled and measured individually by the application-specific integration circuit (ASIC). The wells are inserted into an electrically resistant polymer membrane supported by an array of microscaffolds connected to a sensor chip. In addition to controlling translocation speed, the motor protein has helicase activity, enabling double-stranded DNA or RNA–DNA duplexes to be unwound into single-stranded molecules that pass through the nanopore.Ī MinION flow cell contains 512 channels with 4 nanopores in each channel, for a total of 2,048 nanopores used to sequence DNA or RNA. Changes in the ionic current during translocation correspond to the nucleotide sequence present in the sensing region and are decoded using computational algorithms, allowing real-time sequencing of single molecules. Translocation speed is controlled by a motor protein that ratchets the nucleic acid molecule through the nanopore in a step-wise manner. In an electrolytic solution, a constant voltage is applied to produce an ionic current through the nanopore such that negatively charged single-stranded DNA or RNA molecules are driven through the nanopore from the negatively charged ‘ cis’ side to the positively charged ‘ trans’ side. The technology relies on a nanoscale protein pore, or ‘nanopore’, that serves as a biosensor and is embedded in an electrically resistant polymer membrane 1, 3 (Fig. ![]() The CIC also supports the teaching missions of Biology and Biomedical Engineering curricula and provides service to users from local biotech companies.Nanopore sequencing technology and its applications in basic and applied research have undergone substantial growth since Oxford Nanopore Technologies (ONT) provided the first nanopore sequencer, MinION, in 2014 (refs. The CIC serves the light microscopy needs of Drexel researchers from a variety of academic colleges and departments including the College of Arts and Sciences, the College of Engineering, the School of Biomedical Engineering, and the College of Medicine. The Cell Imaging Center (CIC) is a multi-user facility housed in purpose-built imaging suites in the Papadakis Integrated Sciences Building (PISB) and in the Bossone Research Center. Our mission is to provide a state-of-the-art facility, expertise, and education in advanced quantitative light microscopy to support Drexel University’s research community The CIC ![]() ![]() Google Chrome became the most popular and number one browser not only for Windows but also for Mac but its users cannot use the app without running into some kind of issues.
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